Through CD8+ T cell killing assays, we found that HKDC1 KD could sensitize Hep3B cells to cytolysis by CD8+ T cells (Fig. 1g), while flow-cytometric analysis showed that CD8+ T cells co-cultured with HKDC1 KD Hep3B cells displayed lower PD-1 and LAG-3, but higher IFNγ and GzmB expression (Fig. 1h, i), indicating tumor cell-mediated immune suppression was alleviated by HKDC1 depletion. This evidence concerns the gene LAG3 and neoplasm.