In an HCC mouse model established by hepatic portal vein injection of mHKDC1 KD (shmHKDC1 targeting 3′-UTR of mHKDC1 transcripts) Hepa1-6 cells in immune-competent C57BL/6 mice, we found that overexpression of either mHKDC1WT or mHKDC1S600A could largely restore Hepa1-6 tumor growth suppressed by shmHKDC1, suggesting the role of HKDC1 in promoting tumor progression independent of its hexokinase activity in immune-competent mice (Fig. 2c and Supplementary Fig. 3a). Here, HKDC1 is linked to neoplasm.