Indeed, qPCR analysis of Cd274 expression in tumor tissues from HCC model mice used in Fig. 2C showed that mHKDC1 KD significantly reduced Cd274 transcription, while either mHKDC1WT or mHKDC1S600A overexpression resulted in similar Cd274 expression to that in the NTC controls (Supplementary Fig. 3j). This evidence concerns the gene CD274 and hepatocellular carcinoma.