From an immunophenotyping point of view, based on the analyses performed on the samples characterized when available, it was apparent that all the classic NB markers (CD56, GD2, B7-H3) and the recently discovered cell surface receptor nucleolin (NCL) [45], are maintained along the different PDX generations and the PDX-derived 3D spheroids. This evidence concerns the gene NCAM1 and neuroblastoma.