MAS1 and lactic acidosis: The overdose of APAP is linked to lactic acidosis, oxidative stress, and mitochondrial dysfunction.[12, 21] Considering the documented influence of Mas on hepatocyte metabolism in mouse AILI and the significant contribution of metabolic status to cellular function, we analyzed the metabolic characteristics of ECs, which predominantly rely on glycolysis.[22] The glycolytic activity of EC7 was the highest among all clusters (Figure 2A), which exhibited a significant upregulation in the EC transition trajectory (Figure S4, Supporting Information).