Overexpression of miR-22-3p attenuated asthma in mice by regulating NLRP3-caspase-1-IL-1β axis.62 Besides, miR-22-3p could directly target NLRP3 according to dual-luciferase reporter assay.63 Studies found that miR-214-3p contains caspase-1-binding sites, overexpression of miR-214-3p decreased caspase-1 levels in fibroblasts.64 Another study also verified that transfection of miR-214-3p reduced CASP1 activity by luciferase assay and decreased gene expression of CASP1 by qPCR experiment.65 MiR-221-5p regulated inflammatory responses in acute gouty arthritis, which can target IL1B gene. The gene discussed is NLRP3; the disease is gout.