The iGTT showed prolonged hyperglycemia in WT females fed HFD, while cKOTw2 had significantly lower blood glucose levels (Supplemental Figure 4, E and F), thus reversing the trend seen in female mice kept on regular chow diet (see above); however, both mutant and WT females responded equally to an insulin bolus (Supplemental Figure 4, G and H). This evidence concerns the gene INS and Hyperglycemia.