HeLa cells were transfected with Arf1-WT, Arf1-Q71L, and Arf1-T31N as HA fusion proteins before infection with Tg-mCh. At 48 hpi, the cells were fixed, stained with anti-HA, and visualized by microscopy (Fig. 2A; Fig. S5A). This evidence concerns the gene ARF1 and infection.