The above variables were subsequently included in multivariate Cox regression analysis, and the results showed that beyond Milan criteria, pre-transplant AFP greater than 400, poor tumour differentiation, microvascular invasion and HBV reactivation were independent risk factors for post-transplant LMFS (Supplemental Table 3, Supplemental Digital Content 6, http://links.lww.com/JS9/B894). This evidence concerns the gene AFP and neoplasm.