Seven weeks of high-intensity interval training downregulates hippocampal cAMP response element binding protein (CREB) phosphorylation and brain-derived neurotrophic factor (BDNF) protein levels, upregulates the protein expression of mitochondrial respiration Complex III, Complex IV, and Complex V, reduces those of Complex I, LC3-II and Bnip3L and LC3-II/I ratio, and inhibits hippocampal mitophagy, which in turn decreased spontaneous behavior and exploratory activities and increases anxiety levels in middle-aged mice (Zhang Y. et al., 2021). This evidence concerns the gene BDNF and Anxiety.