Eight weeks of aerobic endurance exercise and choline injection intervention increase myocardial M2Acetylcholine receptor (M2AChR) expression in TAC surgery-induced cardiac hypertrophy mice, followed by downregulation of Mfn1, Mfn2, Drp1, LC3, BNIP3, and Parkin proteins, with no significant changes in the levels of the OPA1 protein. This evidence concerns the gene MAP1LC3A and cardiac hypertrophy.