Moreover, it activated the AMPK/SIRT1/PGC-1α pathway, thereby promoting mitochondrial biogenesis in NAFLD zebrafish, while upregulating the levels of PINK1 and Parkin proteins, decreasing the expression of p62, and promoting the level of mitophagy, which then significantly reduced the level of collagen type I α1 chain (COL1A1), α-actinin-2 (ACTA2), and IL-β expression in NAFLD zebrafish liver, and increased the expression of anti-inflammatory cytokine IL-10. This evidence concerns the gene SQSTM1 and metabolic dysfunction-associated steatotic liver disease.