Que significantly increased liver ATG5, ATG12, LC3, PINK1, and Parkin protein levels in NAFLD mice induced by methionine and choline deficiency (MCD) diet, promoted mitophagy, inhibited nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) protein levels, reduced serum alanine aminotransferase (ALT)/aspartate aminotransferase (AST) and ROS levels, and alleviated hepatic atrophy and lipid accumulation in NAFLD mice. This evidence concerns the gene ATG12 and metabolic dysfunction-associated steatotic liver disease.