After data cleaning and reduction and grouping, the TRIM56 positive cells were divided into glioma cells (expressing SOX2, OLIG1, GFAP, and EGFR), myeloid cells (mainly macrophages and microglia, expressing PTPRC and CD68), T cells (expressing PTPRC, CD3E, and CD3D), oligodendrocytes (expressing MBP and MOG), NK cells (expressing PTPRC, FGFBP2, and NKG7), B cells (expressing PTPRC, CD79A, and CD79B), endothelial cells (expressing VWF and PECAM1), and pericytes (expressing ACAT2 and PDGFRB) according to specific cell markers (Figures 7A, B). The gene discussed is CD3D; the disease is glioma.