Our results represented significant depression of GSDMD, GSDMD-N, IL-1β and IL-18 when MCC950 was used to inhibit the level of NLRP3 inflammasome in kidneys of I/R-induced AKI, which confirmed that NLRP3-inflammasome mediated pyroptosis caused by renal I/R injury, as supported by other studies [14, 17, 19]. This evidence concerns the gene GSDMD and acute kidney injury.