According to current data, it showed that tubules pyroptosis acted as a promoter in renal I/R injury and inhibition of NLRP3-mediated pyroptosis alleviated severity of AKI after I/R; Cx32 played a regulatory role in AKI and NLRP3-related pyroptosis, as element protein of GJ among renal cells, and meanwhile it enhanced the signaling of miR155-3p after I/R or H/R. Here, GJB1 is linked to acute kidney injury.