In a unilateral ureteral obstruction (UUO) mouse model, activation of Sirt1 signaling was accompanied by an increase in phosphorylated endothelial nitric oxide synthase (eNOS) levels, and Sirt1 interacted with eNOS to improve the UUO model for scoring renal fibrosis [38]. The gene discussed is SIRT1; the disease is renal fibrosis.