Remarkably, the protein expression levels of HMGB1 and acetyl-K29-HMGB1 were increased in CRC tissues with the upregulation of MSI2 (Fig. 7F), and the protein expression levels of MSI2 and HMGB1 were highly positively correlated in our clinical CRC specimens (R = 0.66, p = 1.7e-07) (Fig. 7H) and CPTAC Colon cancer dataset (R = 0.39, p = 9.7e-05) (Fig. 7J). Here, MSI2 is linked to colonic neoplasm.