In addition, the protein levels of P300, HMGB1 and K29-HMGB1 and NF-κB signaling pathway components in Msi2Transgenic CAC mice and stable CRC cell lines were also examined, and MSI2 was found to increase the protein levels of P300, HMGB1, K29-HMGB1 and p-p65 (Fig. 4Q-R and Fig. S8F). This evidence concerns the gene HMGB1 and colorectal carcinoma.