The top 100 upregulated (including ATPase Phospholipid Transporting 8A2 [ATP8A2]) and downregulated genes (including UTY, KDM5D, INS, NLGN4Y, TXLNG, EIF1AY, ZFY, TAF11L12, CACNA2D1-AS1, USP9Y) for each ICC sample were identified (Fig. 3c). The gene discussed is CACNA2D1; the disease is intrahepatic cholangiocarcinoma.