After cell segmentation, we were able to classify each cell type (ANTXR1+ and ANTXR1- CAF, CD8+ T lymphocytes and pan-cytokeratin+ cancer cells) based on intensities of their specific proteins using an unsupervised approach (Fig. 5F, G) (See also Methods #Pan-cytokeratin, CD8 and ANTXR1 multiplex immunofluorescence imaging and colocalization analysis). Here, CD8A is linked to cancer.