Culturing U87MG (Supplementary Fig. S3a) and mouse neuroblastoma (Neuro-2a) (Supplementary Fig. S3b) cells in low-glucose medium failed to induce changes in SIRT4 and GAD1 protein and mRNA levels, ruling out the possibility that the KD drives SIRT4 overexpression solely owing to its low carbohydrate content. This evidence concerns the gene GAD1 and neuroblastoma.