Reflecting on the fact that wild-type HNSCC cells demonstrate a decrease in p62 level and no visible changes by western blot in LC3B-II after mTORC1 inhibition alone, without CQ treatment, while NSD1-depleted cells have different profiles of p62, LC3B-II, and ULK1 protein levels, suggested that the effects we observe reflect an impaired autophagic flux upon NSD1 depletion. This evidence concerns the gene ULK1 and head and neck squamous cell carcinoma.