Using the affinity-purified K20me antibodies, we examined K20 methylation of EZH2 in human breast ductal carcinoma T47D cells, as this cell line was previously used to analyze the effect of AKT-mediated serine phosphorylation in EZH2 (Cha et al., 2005). Here, AKT1 is linked to breast ductal adenocarcinoma.