To further examine whether loss of KDM1A affects the protein stability of wild-type EZH2, K20R, and S21A mutant EZH2 proteins, human rhabdoid tumor G401 cells stably expressing the HA-tagged wild-type EZH2, EZH2K20R, and EZH2S21A were established and these cells were transfected with Kdm1a siRNA, followed by treating them with protein synthesis inhibitor, cycloheximide, to block translational initiation to measure protein decay rates (Guo et al., 2022). Here, EZH2 is linked to rhabdoid tumor.