The significantly upregulated differentially expressed genes in AD intersected with the neurotoxic astrocyte cell population, resulting in the identification of twelve key genes, including GNG12, ZFP36L1, AEBP1, WWTR1, PHYHD1, RASL12, DST, CUL4A, ADAMTSL3, LTBP1, NKTR, ANGPT1 (Fig. 3D, Table 2). This evidence concerns the gene CUL4A and Alzheimer disease.