The significantly upregulated differentially expressed genes in AD intersected with the neurotoxic astrocyte cell population, resulting in the identification of twelve key genes, including GNG12, ZFP36L1, AEBP1, WWTR1, PHYHD1, RASL12, DST, CUL4A, ADAMTSL3, LTBP1, NKTR, ANGPT1 (Fig. 3D, Table 2). The gene discussed is WWTR1; the disease is Alzheimer disease.