As shown in the waterfall plot (Figure 6A, 6B), there are variations in the tumor mutational burden (TMB) of two subtypes, and the frequencies of TTN, CSMD3, MUC16, RYR2, LRB1P and ZFHX4 mutations in the high-risk group were considerably higher than those in the low-risk group. The gene discussed is CSMD3; the disease is neoplasm.