In a model of STZ-induced DN in rats receiving a diet high in sugar and fat, Chinese herbal granules (containing leeches) were found to increase the expression of podocyte α-actinin-4, Synaptopodin protein, and cleavage proteins podocin and CD2AP, while also reducing levels of 24-hour urinary protein (24h-Upro) in DN rats (39, 40). This evidence concerns the gene CD2AP and liver dysplastic nodule.