Given the various reasons for the absence of bi-allelic inactivating of MMR genes [e.g., assay limitations, epigenetic or complex structural alterations, technical issues, insufficient coverage, low tumor purity, or poor mapping quality particularly for PMS2, or age-related LOH contribution typically observed in younger patients (13)], these scenarios were excluded from the INT2GRATE POSITIVE criteria. The gene discussed is PMS2; the disease is neoplasm.