CDC42 and Hyperglycemia: In conclusion, this study indicates that hyperglycemia induces the overexpression of cdc42 and overactivation of the downstream effector N-WASP/Arp2/3 signaling to promote the polymerization of G-actin into F-actin, which is located at the edge of the cell, resulting in increased stiffness of cytoskeleton, destruction of the polarity and reduction in the migration of HUVECs.