In addition, the mutant mice group exhibited gradually increasing bodyweight from 3 months to 12 months of ages, however, marked variations in bodyweights were observed in mutant mice between 6 and 12 months of age compared to that in the sham group (Supplementary Fig. S2), possibly due to the Tdp-43 pathology-associated movement disorders, resulting in either obesity [44] or malnutrition. Here, TARDBP is linked to obesity due to melanocortin 4 receptor deficiency.