We adopted Differential NicheNet (33), an extension of the default NicheNet algorithm, to depict L-Rs specific for CD8.c05 and CD8.c11 post-ICIs, mainly focused upon ligands from CD4+ T cells in MPR-E and PD1-R, given the widely accepted concept of the delicate interplay between CD4+ and CD8+ T cells in the tumor immune cycle. The gene discussed is CD4; the disease is neoplasm.