CD4 and neoplasm: The in vitro transcriptomics analysis revealed that HIFU irradiation enriched some differentially expressed genes in the NF‐kappa B pathway (Figure 4A), which cooperatively induces the expression of IL1‐type cytokines and promotes the antitumor activity of both CD4 and CD8 T cells.[28] Interestingly, it was found that HIFU and NP‐G/P+H treatments significantly increased IL1 expression in tumors compared with PBS treatment (Figure 6C), indicating HIFU helps to reshape the tumor immune microenvironment.