The majority of amplicons contained the RNU2‐1 fragments containing additional GAGA nucleotides (69%) but sequences complementary to the precursor of miR‐1246 could not be detected (Figure 4C), consistent with previous findings.[34] We introduced siRNA to knock down RNU2‐1 level in CRC cells, but due to the constitutive expression of RNU2 transcript in eukaryotic cells, it was challenging to suppress RNU2‐1 expression using siRNA. The gene discussed is RNU2-1; the disease is colorectal carcinoma.