The M1W‐TCR with its corresponding mutation library (mixed with 0.1% WT M1W‐TCR virus) was transduced into CD8‐expressed J76 cells (referred to as M1W‐TCR pools‐J76), an endogenous TCRα and TCRβ deficient Jurkat cell, at a multiplicity of infection (MOI) of 4 to achieve a high infection rate (Figure S7D, Supporting Information). The gene discussed is CD8A; the disease is infection.