TMEM65 mRNA was significantly overexpressed in GC tissues as compared with normal tissues in cohort I by Affymetrix chip assay (adjacent normal, n = 24; GC, n = 78; P < 0.001) and TCGA cohort by RNA-seq and normalized as Fragments Per Kilobase of transcript per Million mapped reads (FPKM) (adjacent normal, n = 32; GC, n = 375; P < 0.001), and was confirmed in paired GC tumor tissues as compared to adjacent normal controls in cohort I (n = 24; P < 0.001) (Fig. 1A). The gene discussed is TMEM65; the disease is gastric cancer.