We injected VNP-siTMEM65 or VNP-siNC into the xenograft tumor in the experimental group and the control group, the results showed that targeting depletion of TMEM65 by VNP-siTMEM65 in GC cells significantly reduced tumor volume (P < 0.001; Fig. 4F) and tumor weight (P < 0.01; Fig. 4F). This evidence concerns the gene TMEM65 and neoplasm.