Furthermore, knockdown of SPOP (Supplemental Fig. 5a) significantly reversed the promotional impact of BCLAF1 on HCC cell proliferation (Supplemental Fig. 5b–f), migration (Supplemental Fig. 5g–l), and invasion (Supplemental Fig. 5m–o), suggesting that BCLAF1 is partially dependent on SPOP to promote the malignant phenotype of HCC. Here, SPOP is linked to hepatocellular carcinoma.