Meanwhile, we performed transient overexpression of BCLAF1-WT or BCLAF1-mSBC in SPOP-overexpressing HCC cell lines, respectively, and observed that BCLAF1-WT significantly reversed the modulation of apoptosis, cell cycle, and cytokine levels in Jurkat cells induced by SPOP overexpression, whereas BCLAF1-mSBC group lost these effects (Fig. 7d–k and Supplemental Fig. 7b–i). This evidence concerns the gene SPOP and hepatocellular carcinoma.