Finally, RNA sequencing found that transient stroke induction increased expression levels of GPR4 and TDAG8 but not OGR1, and OGR1 deletion did not affect expression levels of GPR4 or TDAG8 under control or ischaemic conditions, suggesting that OGR1 does not affect GPR4 or TDAG8 expression [103]. Here, GPR4 is linked to stroke disorder.