In the intervention side tumor, the statistical analysis of CD8+T cell levels showed that compared with the Blank group, the Anti PD-1 group, the RT (2 GyX4)+anti PD-1 group, and the RT (8 Gy)+anti PD-1 group all showed a certain level of high expression (p = 0.0029; p = 0.0022; p = 0.0009), and there was no difference between the three treatment groups. The gene discussed is CD8A; the disease is neoplasm.