CD8A and neoplasm: Compared to the RT (8 Gy) group and the RT (2 GyX4)+anti PD-1 group, the level of IFN- γ+ CD8+T cells showed significant differences (p < 0.0001; p = 0.0009), indicating a significant immune regulatory effect after increased radiation therapy, activating the local release of IFN- γ in the tumor.