In highly invasive breast cancer cell lines MCF-7-I4 and MDA-MB-453-I4 derived from MCF-7 and MDA-MB-453 cell lines, respectively [99], Cheng et al. were able to demonstrate that inhibition of STAT3 with small molecule inhibitor JSI-124 reduced pSTAT3 levels with a marked reduction in Twist protein expression [100]. The gene discussed is STAT3; the disease is breast cancer.