We also show that CBLL1, LGR5, and c-MYC mRNA levels are highly upregulated in cancer stem cell tumourspheres compared to monolayer cell cultures, while silencing CBLL1 in cancer stem cell tumourspheres results in a significant reduction in the size of the tumoursphere, which is accompanied by the downregulation of stem cell biomarkers. The gene discussed is CBLL1; the disease is cancer.