CBLL1 silencing was confirmed at the mRNA level (Figure 5b), and mRNA downregulation of other known stem cell markers including LGR5, NANOG and c-MYC was also detected in sh-CBLL1 HT29 cancer stem cell tumourspheres compared to sh-control tumourspheres (Figure 5c–e). This evidence concerns the gene MYC and cancer.