To understand the potential molecular mechanism by which CBLL1 might be involved in cancer stem cells, HCT116 colon cell lines were grown in 2D as a monolayer cell culture (Figure 4a, left panel) and as a 3D cancer stem cell tumoursphere-derived HCT116 (Figure 4a, right panel) by culturing in ultra-low attachment plates grown in a specific medium to enrich cancer stem cell growth. Here, CBLL1 is linked to cancer.