In our study, we found that rosavin was able to eliminate acute lung injury by inhibiting the activation of the TLR-4/NF-κB/MAPK signaling pathway and apoptosis, which not only provides theoretical ideas for elucidating the possible molecular mechanisms of rosavin but also provides a rationale for the potential clinical application of rosavin in the future treatment of ALI. This evidence concerns the gene TLR4 and acute respiratory distress syndrome.