In this study, based on the in vivo and in vitro LPS-induced ALI model, we found that rosavin was able to eliminate lung inflammation by inhibiting the activation of the TLR-4/NF-κB/MAPK signaling pathway and apoptosis, which not only provides theoretical ideas for elucidating the possible molecular mechanism of rosavin but also provides a basis for the potential clinical application of rosavin in the future treatment of ALI. Here, NFKB1 is linked to acute respiratory distress syndrome.