Although the caspase-3 increased and then decreased at 48 h, in the present study, after the transplantation of HUVEC mitochondria, B16F10 cells exhibited enhanced ERK and AKT signaling and increased cyclin D1 and cyclin E expression, suggesting that the transfer of endothelial mitochondria from the TME supports the growth of melanoma, a finding corroborated by relevant studies. This evidence concerns the gene CCNE1 and melanoma.