Several studies reported a dose-dependent cytotoxic effect of sorafenib, along with the induction of the assembly of the autophagolysosome, which was accompanied by reduced cell viability and an increase in annexinV/Pi-positive cells in vitro, as well as by an increase in LC3-II conversion and autophagosome formation in tumor samples and a reduced tumor burden in HCC-bearing mice [37,41,74]. Here, ANXA5 is linked to hepatocellular carcinoma.