Deeper analyses of autophagic events demonstrated that metformin could enhance LC3B mRNA induction and increase the levels of BECN1, GABA type A receptor-associated protein like 2 and LC3-II proteins, accompanied by a reduction in p62 [117] and an increased signal related to LC3B puncta formation [41,112,118,119] and autophagic vacuoles in different HCC cell lines [41,115] and in gastric cells [111]. This evidence concerns the gene BECN1 and hepatocellular carcinoma.