Another study [111] reported that metformin failed to trigger the autophagic flux in two HCC lines and that the type of metformin-induced cell death was different in the two cell lines, mainly due to differences in the basal levels of autophagy potentially related to the activation status of AKT, an upstream regulator of the mTOR complex [115]. The gene discussed is MTOR; the disease is hepatocellular carcinoma.