Therefore, targeted gene sequencing analysis was performed using custom panels focusing on the exome regions of 21 lipid-associated genes, including <i>ABCG5</i>, <i>ABCG8</i>, and familial hypercholesterolemia-causing genes (<i>LDL receptor</i>, <i>LDLRAP1</i>, <i>PCSK9</i>, and <i>apolipoprotein B</i>). This evidence concerns the gene PCSK9 and familial hypercholesterolemia.