To confirm that the inhibition of VEGF was due to the downregulation of HIF-1-α, a VEGF promoter reporter and HIF-1-α plasmids were transfected into OVCAR-3 cells, and the inhibition of VEGF was reversed due to the constitutive expression of HIF-1-α, confirming that angiogenesis in ovarian cancer cell lines is dependent on the expression of HIF-1-α [98]. The gene discussed is HIF1A; the disease is ovarian cancer.