Peng et al. further displayed through quantitative polymerase chain reaction (qPCR) the inhibition of microRNA-374a (miR-374a) messenger RNA (mRNA) by ISL dose-dependently and upregulation of PTEN mRNA 15-fold in breast cancer cells (MCF-7 and MDA-MB-231) after 24 h [108]. This evidence concerns the gene PTEN and breast cancer.