Interestingly, we showed that murine 20D7SL CAR-T cells effectively control tumor growth with or without IL-2 supplement (Fig. 3g–i, Supplementary Fig. 2g–h), even in the highly aggressive B16 murine tumor model that does not respond to PD-1 blockade due to immune exclusion mechanisms31. Here, PDCD1 is linked to neoplasm.