In addition, necroptosis and ferroptosis may be the mechanisms underlying melanoma cell death simultaneously; for example, BAY 87-2243-induced melanoma cell death due to ROS accumulation through mitochondrial complex I inhibition was attenuated by necrostatin (a necroptosis inhibitor), knockdown of RIPK1 or MLKL, ferrostatin (a ferroptosis inhibitor) or knockdown of GPX4 but not treatment with the pancaspase inhibitor z-VAD-FMK [97]. This evidence concerns the gene RIPK1 and melanoma.