To investigate the effector function of our TCR-Ts against EC, we set up in vitro cytotoxicity and IFNγ release assays where we co-cultured our TCR-Ts with varying cancer cell lines or TCR-Ts with T2 cells pulsed with various antigenic peptides at E:T ratios 1:1 or 2:1. This evidence concerns the gene IFNG and cancer.