Wu et al’s GSEA (Gene set enrichment study) revealed a significant link between AQP1 up-regulation and the TGF- signaling pathway in osteosarcoma, and the production of TGF-β1 and TGF-β2 was decreased in U2OP and MG63 cell lines when AQP1 expression was lowered by shRNA [31].. This evidence concerns the gene AQP1 and osteosarcoma.