PRF1 and melanoma: Leveraging The Cancer Genome Atlas (TCGA) datasets, we investigated NR4A1 expression in melanoma patients and identified a negative correlation between NR4A1 and genes involved in the antitumor immune responses, including IFNγ (interferon γ), GZMB (granzyme B), and PRF1 (perforin) (Fig. S1, A–C).