One study showed that overexpressed SPRY4-IT1 can up-regulate the expression of Twist1 and Vimentin, and inhibit transcription of E-cadherin, thereby promoting the EMT process of HCC tissue, enhancing the growth and invasive ability of HCC, and increasing the probability of extrahepatic metastasis of HCC [102]. This evidence concerns the gene SPRY4 and hepatocellular carcinoma.