For this purpose, mice (n = 6/group) inoculated (s.c.)with Mia PaCa-2 cells were treated with increasing amounts of compound XII (daily injections of 20, 40, or 80 mg/kg) or vehicle alone, as described in Figure 2A. On Day 25, the tumors were resected and the tumor tissue lysates were subjected to Western blotting, applying anti-heparanase and anti-GAPDH polyclonal antibodies. This evidence concerns the gene HPSE and neoplasm.