TP53 and colorectal carcinoma: To ensure that the RGNPs were capableof delivering their payloadintracellularly, a NP uptake study was conducted using a fluorescentRhodamine B-conjugated PLGA polymer, which was blended into the formulationto generate a NP that was trackable by flow cytometry and microscopyin a range of exemplar p53 wild-type CRC cell lines (RKO, LoVo, andHCT116 p53+/+).