Similarly, we measured the double-positive staining area of PR/PDGFRα and PR/c-KIT by using lasx software, and found that the expression of PR co-located with PDGFRα+ cells was more (Fig. 5E; n = 5; P < 0.05) and the expression of PR co-located with ICC was less (Fig. 5F; n = 6; P < 0.05) in pregnant mice group compared with the non-pregnant control group. Here, PGR is linked to intrahepatic cholangiocarcinoma.