The findings demonstrated that NF-κB p65 and IL-1β expression was elevated in the LPS group, implying that the ALI mouse model had been established (P<0.001), and their expression was decreased after treatment with 25 μl of CON-exos (P<0.05 to P<0.01, Figure 5E, F).Consequently, volume of 25 μl of CON-exos was selected in the follow-up experiments in vivo. Here, NFKB1 is linked to acute respiratory distress syndrome.