Pyroptosis-related genes (NLRP3, PYCARD, Caspase-1, IL-1β and Cleaved IL-18) were discovered to be upregulated in calcific valves, and a suppressor of NLRP3 could alleviate pyroptosis and CAVD progression (15). Here, NLRP3 is linked to congenital bilateral aplasia of vas deferens from CFTR mutation.