The protein level of ALKBH7 and APNG was detected by Western blot (Figure 6F), which disclosed that reduced APNG expression in the co‐cultured glioblastoma cells after ALKBH7 knockdown, which implied that APNG expression suppressed cytotoxic effect of TMZ, ALKBH7‐dependent APNG expression was a critical step for TMZ resistance of glioblastoma cells. This evidence concerns the gene MPG and glioblastoma.